Analytical Methods

Colorimetric aptasensor based on temporally controllable light-stimulated oxidase-mimicking fluorescein for the sensitive detection of exosomes in mild conditions

A colorimetric aptasensor for exosome detection under LED light.

Immunoaffinity-free chromatographic purification of ovarian cancer biomarker CA125 (MUC16) from blood serum enables mass spectrometry characterization

The enrichment of trace proteins from human fluid samples is of great importance in diverse clinical and industrial applications.

Advances in human papillomavirus detection for cervical cancer screening and diagnosis: challenges of conventional methods and opportunities for emergent tools

This review evaluates various point-of-care (POC) methodologies for HPV detection and highlights the current advancements in POC diagnostic devices.

Development of an HPV 16 rapid test founded in user-centered design with primary care clinicians

Clinician-informed nucleic acid test for cervical cancer screening to facilitate same-visit results to improve follow-up care.

Digital multimeter-based portable photoelectrochemical immunoassay with enzyme-catalyzed precipitation for screening carbohydrate antigen 125

A photoelectrochemical immunoassay was devised to detect CA-125 employing enzyme-catalyzed precipitation on hollow porous In2O3 nanotubes with CdS nanoparticles.

Photonic crystal barcodes assembled from dendritic silica nanoparticles for the multiplex immunoassays of ovarian cancer biomarkers

The combined detection of CA125, CEA and AFP is of great significance in the diagnosis of ovarian cancer.

Rapid detection of HPV16/18 based on a CRISPR-Cas13a/Cas12a dual-channel system

Orthogonal cleavage of DNA and RNA reporters by target-activated Cas12a/Cas13a induces two-colored fluorescence emission (JOE-FAM), which can be detected using a standard fluorometer or observed by a dual-channel visualization detection platform.

Evaluating the effectiveness of whole blood plasma versus protein precipitates in ovarian cancer detection through infrared spectroscopy

ATR-FTIR spectroscopy to analyse blood plasma samples from benign and ovarian cancer cases. Accuracy values of 96% and 92% were achieved for the protein precipitates and whole plasma datasets, respectively.

Multimodal detection of high-risk HPV oncogenic transcripts using a branched hybridization chain reaction amplification method

A branched hybridization chain reaction amplification method for multimodal detection of high risk HPV oncogenic transcripts. The bHCR assay is a versatile diagnostic strategy with adaptability to other molecular biomarkers.

Development of a highly sensitive, label-free electrochemical immunosensor for p16INK4a detection: a step toward early cervical cancer diagnosis

A gold-reduced graphene oxide hybrid electrochemical immunosensor was developed for ultrasensitive detection of the p16INK4a protein, showing promise for the early diagnosis of cervical cancer in clinical samples.

Rapid detection of HPV16 utilizing recombinase polymerase amplification with the employment of an extremely low concentration of the probe

Human papillomavirus 16 (HPV16) infection is the leading cause of cervical cancer.

Rapid immunoassay for dual-mode detection of HPV16 and HPV18 DNA based on Au@PdPt nanoparticles

A lateral flow immunoassay (LFIA) based on Au@PdPt NPs was developed to detect and type the HPV16 and HPV18 simultaneously.

Construction of a hydrophilic porphyrin-based MOF@COF hybrid via post-synthetic modification for N-glycopeptides analysis in human serum

This work introduces the analysis of trace N-glycopeptides in human serum by a hydrophilic porphyrin-based MOF@COF hybrid.