Protocol for generating an in vitro 3D multicellular culture model of ovarian high-grade serous carcinoma

Lidia Moyano-Galceran · 2026-02-23

The development of translational ovarian cancer models to investigate and overcome treatment resistance, while accounting for the impact of the tumor microenvironment, is critical. Here, we present a protocol to establish a multicellular culture model that retains both genetic complexity and the microenvironment of patient tumors and is amenable to molecular and phenotypic analyses and high-throughput drug testing. We describe steps for culturing and characterizing stromal cells derived from cryopreserved and fresh samples and detail procedures for combining them with organoids.
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DOIPubMed
Journal
STAR Protocols
TL;DR

A protocol to establish a multicellular culture model that retains both genetic complexity and the microenvironment of patient tumors and is amenable to molecular and phenotypic analyses and high-throughput drug testing is presented.

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Institutions
Karolinska Institutet
Authors
Lidia Moyano-Galceran
Funding
Novo Nordisk Foundation Grant NNF21OC0070381Innovation Fund Denmark Grant /ERA PerMed JTC2020Innovation Fund Denmark Grant 0204-00005BEU Framework Programme for Research and Innovation Marie Skłodowska-Curie Actions Grant 101063359Danish Cancer Society Grant R374-A22457Horizon 2020 Framework Programme Funding