Investigator

Abhishek Jain

Associate Professor · Texas A&M University, Biomedical Engineering

Research Interests

AJAbhishek Jain
Papers(3)
Angiogenesis‐Enabled …OvCa-Chip microsystem…Human tumor microenvi…
Collaborators(8)
Biswajit SahaTanmay MathurVahid Afshar-KharghanAkhilesh K. GaharwarJames J. TronoloneGiriraj K. LokhandeAnil K. SoodGang Bao
Institutions(4)
Houston MethodistUncncsu Joint Departm…The University Of Tex…Rice University

Papers

Angiogenesis‐Enabled Human Ovarian Tumor Microenvironment‐Chip Evaluates Pathophysiology of Platelets in Microcirculation

AbstractThe tumor microenvironment (TME) promotes angiogenesis for its growth through the recruitment of multiple cells and signaling mechanisms. For example, TME actively recruits and activates platelets from the microcirculation to facilitate metastasis, but platelets may simultaneously also support tumor angiogenesis. Here, to model this complex pathophysiology within the TME that involves a signaling triad of cancer cells, sprouting endothelial cells, and platelets, an angiogenesis‐enabled tumor microenvironment chip (aTME‐Chip) is presented. This platform recapitulates the convergence of physiology of angiogenesis and platelet function within the ovarian TME and describes the contribution of platelets in promoting angiogenesis within an ovarian TME. By including three distinct human ovarian cancer cell‐types, the aTME‐Chip quantitatively reveals the following outcomes—first, introduction of platelets significantly increases angiogenesis; second, the temporal dynamics of angiogenic signaling is dependent on cancer cell type; and finally, tumor‐educated platelets either activated exogenously by cancer cells or derived clinically from a cancer patient accelerate tumor angiogenesis. Further, analysis of effluents available from aTME‐Chip validate functional outcomes by revealing changes in cytokine expression and several angiogenic and metastatic signaling pathways due to platelets. Collectively, this tumor microphysiological system may be deployed to derive antiangiogenic targets combined with antiplatelet treatments to arrest cancer metastasis.

OvCa-Chip microsystem recreates vascular endothelium–mediated platelet extravasation in ovarian cancer

Abstract In ovarian cancer, platelet extravasation into the tumor and resulting metastasis is thought to be regulated mostly by the vascular endothelium. Because it is difficult to dissect complex underlying events in murine models, organ-on-a-chip methodology is applied to model vascular and platelet functions in ovarian cancer. This system (OvCa-Chip) consists of microfluidic chambers that are lined by human ovarian tumor cells interfaced with a 3-dimensional endothelialized lumen. Subsequent perfusion with human platelets within the device’s vascular endothelial compartment under microvascular shear conditions for 5 days uncovered organ-to-molecular–level contributions of the endothelium to triggering platelet extravasation into tumors. Further, analysis of effluents available from the device’s individual tumor and endothelial chambers revealed temporal dynamics of vascular disintegration caused by cancer cells, a differential increase in cytokine expression, and an alteration of barrier maintenance genes in endothelial cells. These events, when analyzed within the device over time, made the vascular tissue leaky and promoted platelet extravasation. Atorvastatin treatment of the endothelial cells within the OvCa-Chip revealed improved endothelial barrier function, reduction in inflammatory cytokines and, eventually, arrest of platelet extravasation. These data were validated through corresponding observations in patient-derived tumor samples. The OvCa-Chip provides a novel in vitro dissectible platform to model the mechanisms of the cancer-vascular-hematology nexus and the analyses of potential therapeutics.

44Works
3Papers
8Collaborators
NeoplasmsThrombosisVenous ThrombosisAtherosclerosisTumor MicroenvironmentOvarian NeoplasmsCell Line, TumorVascular Diseases

Positions

2022–

Associate Professor

Texas A&M University · Biomedical Engineering

2016–

Assistant Professor

Texas A&M University · Biomedical Engineering

2015–

NIH NRSA Award Research Fellow

Harvard University · Wyss Institute for Biologically Inspired Engineering

2012–

Research Fellow

Harvard University · Wyss Institute for Biologically Inspired Engineering

2008–

PhD Research Assistant

Massachusetts General Hospital · Steele Lab for Tumor Biology

2004–

Research Associate

Universiteit Utrecht · Earth Science

2003–

Trainee - Assistant Manager

Coca Cola

Education

2011

PhD

Boston University · Biomedical Engineering

2007

MS

Arizona State University · Mechanical Engineering

Keywords
MicrofluidicsHemostasis and ThrombosisOrgans-on-ChipBioengineering