HJURP Derived from Cancer-Associated Fibroblasts Promotes Glutamine Metabolism to Induce Resistance to Doxorubicin in Ovarian Cancer
Cancer-associated fibroblasts (CAFs) are closely associated with tumor drug resistance. This study intended to delineate how CAFs induced DOX resistance in ovarian cancer. Differential gene expression analysis of ovarian cancer CAFs was completed using Gene Expression Omnibus database. CAFs and normal fibroblasts (NFs) were isolated from ovarian cancer tissues and adjacent normal tissues. The expressions of Holliday Junction Recognition Protein (HJURP), α-smooth muscle actin (α-SMA), and fibroblast activation protein alpha (FAP) were assessed by quantitative reverse transcription polymerase chain reaction and Western blot (WB), α-SMA and FAP were detected by immunofluorescence. A2780 cells were treated with CAF or NF conditioned medium (CM), and protein expression of HJURP was assessed by WB. A2780-DOX cells were constructed and cultured with CAF or NF CM, and cell viability and IC