Mechanistic role of FN1 in LAIR-1 mediated downregulation of ovarian cancer cell proliferation
RNA-seq was used to explore the potential mechanism underlying human leukocyte-associated immunoglobulin-like receptor 1 (LAIR-1) inhibition of the proliferation and migration of ovarian cancer cells. A LAIR-1-overexpression cell model was established using a LAIR-1-lentivirus. After confirming and identifying the LAIR-1 expression cell clones by flow cytometry and RT-qPCR, the proliferation and migration of the cells were examined by CCK8 and scratch assays, and the differentially expressed genes (DEGs) were searched by RNA-seq and analyzed by GO and KEGG enrichment. String was used for protein interaction network analysis, and Cytoscape was used to identify key proteins. LAIR-1 inhibited the proliferation and migration of ovarian cancer cells. LAIR-1 expression caused the upregulation of 83 genes and the downregulation of 80 genes. Among the DEGs, fibronectin 1 (FN1) was a key protein affecting the downstream FAK-MEK-ERK axis. KEGG enrichment analysis identified the MAPK pathway as the most obvious enrichment pathway, followed by PI3K-AKT pathway. LAIR-1 downregulates FN1 to inhibit the FAK-MEK-ERK axis, as well as the proliferation and migration of ovarian cancer cells.
Journal
BMC CancerAuthors
Funding
National Natural Science Foundation of China Grant 82200109National Natural Science Foundation of China Grant 81672757Science and Technology Support Plan for Youth Innovation of Colleges and Universities of Shandong Province of China Grant 2022KJ090Science and Technology Program of Binzhou Medical University Grant BY2019KYQD25Natural Science Foundation of Shandong Province Grant ZR2021LZY024