Differentially expressed circular RNA profiles and comprehensive analysis of circRNA-miRNA-mRNA regulatory network in microsatellite instability-high endometrial cancer

Weijia Wen & Shuzhong Yao et al. · 2024-08-30

The clinical benefit of anti-programmed cell death protein 1 (PD-1)-based immunotherapy among patients with microsatellite instable (MSI) endometrial cancer (EC) precedes that of microsatellite stable (MSS) EC, the mechanisms of which have not been fully understood. Circular RNAs (circRNAs) were reported to modulate immune evasion in several types of malignancies, while their roles in the immune regulation in EC remain largely unknown. Here, we conducted circRNA array analysis and mRNA-Sequencing of 10 MSI EC samples and 10 MSS EC samples and identified 1083 differentially expressed circRNAs (DE-circRNAs) and 864 differentially expressed mRNAs, based on which we constructed a circRNA-miRNA-mRNA comprehensive network consisting of 35 DE-circRNAs, 56 predicted miRNAs and 24 differentially expressed mRNAs. Finally, we confirmed hsa_circ_0058230 being positively correlated with CD8+ T cells infiltration, suggesting that it might take a part in anti-tumor immunity in EC.
Journal
Genomics
Authors
Weijia Wen, Li Yuan, Xueyuan Zhao, Yan Jia, Linna Chen, Hongye Jiang, Wei Wang, Chunyu Zhang, Shuzhong Yao
Funding

Science and Technology Planning Project of Guangdong Province

Guangzhou Municipal Science and Technology Project

Medical Science and Technology Foundation of Guangdong Province

National Natural Science Foundation of China

China Postdoctoral Science Foundation

Key Technologies Research and Development Program

Basic and Applied Basic Research Foundation of Guangdong Province