Protocol for assessing mobilization of peritoneal B cells to the pre-metastatic omentum in an orthotopic mouse model of ovarian cancer
The omentum is a visceral adipose tissue that undergoes dynamic immunological changes prior to and following metastasis. Here, we present a protocol for assessing the mobilization of peritoneal B cells to the pre-metastatic omentum in a mouse ovarian cancer model. We describe steps for isolation and adoptive transfer of peritoneal donor B cells and their detection in the omentum of recipient mice. This protocol could be utilized to study the mobilization of peritoneal B cells to the omentum in other pathological contexts. For complete details on the use and execution of this protocol, please refer to Lee et al.
Journal
STAR ProtocolsTL;DR
Funding
Pre-metastatic Omental Niche Formation in Ovarian CancerBoosting anti-tumor immunity in the omentum for metastasis preventionWhitehead Institute for Biomedical Research, Massachusetts Institute of Technology FundingUS Department of Defense FundingPre-metastatic Omental Niche Formation in Ovarian CancerBoosting anti-tumor immunity in the omentum for metastasis preventionNational Institutes of Health FundingUS Office of Congressionally Directed Medical Research Programs Grant W81XWH-22-1-0122