Protocol for quantifying drug sensitivity in 3D patient-derived ovarian cancer models

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Kathrin B. Labrosse; Ricardo Coelho; Francis Jacob

doi:10.1016/j.xpro.2024.103274

Three-dimensional (3D) ex vivo cultures allow the study of cancer progression and drug resistance mechanisms. Here, we present a protocol for measuring on-target drug sensitivity in a scaffold-free 3D culture system through quantification of apoptotic tumor cells. We provide detailed steps for sample processing, immunofluorescence staining, semi-high-throughput confocal imaging, and imaged-based quantification of 3D cultures. This protocol is versatile and can be applied in principle to any patient-derived material.

Protocol for quantifying drug sensitivity in 3D patient-derived ovarian cancer models

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