Protocol for real-time monitoring of CD8+ T and myeloid cell behavior in human high-grade serous ovarian cancer slices

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doi:10.1016/j.xpro.2024.103102

Protocol for real-time monitoring of CD8+ T and myeloid cell behavior in human high-grade serous ovarian cancer slices

Florian Laforêts & Frances Balkwill et al. · 2024-06-02

Studying cell behavior in live human tumors is crucial to understand and improve response to immunotherapies. Here, we present a protocol to slice human ovarian tumors ex vivo, maintain their viability for 24 h, and monitor the behavior of CD8

Links

DOI PubMed

Journal

STAR Protocols

Authors

Florian Laforêts, Emmanuel Donnadieu, Frances Balkwill

Funding

Ligue Nationale Contre Le Cancer Grant EL2020.LNCC Barts Cancer Institute Funding GlaxoSmithKline Funding Cancer Research Campaign Grant A16354 Cancer Research Campaign Grant A25714