Oncogenic GINS1 facilitates cervical cancer progression via FYN-mediated EMT regulation
To investigate the expression, function, and mechanism of oncogenic GINS1 in cervical cancer pathogenesis. And to explore the potential of GINS1 as a complementary diagnostic biomarker and therapeutic target for cervical cancer. We performed bioinformatic analysis, in vitro functional assays (proliferation, migration, invasion and colony formation), a cell line-derived xenograft model, and validation in clinical specimens. Transcriptome sequencing, along with biochemical and molecular analyses, were used to explore the mechanism. GINS1 was significantly upregulated in cervical cancer. It was essential for proliferation, migration, invasion, colony formation, and tumorigenesis in vivo. GINS1 promoted EMT and tumor aggressiveness by suppressing FYN. GINS1 protein levels positively correlated with advanced FIGO staging. Our study establishes GINS1 as a novel oncogene driving cervical cancer progression via the FYN/EMT axis, positioning it as a promising complementary diagnostic biomarker and potential therapeutic target.