Immunochromatographic assay for rapid detection of SIGLEC15 to evaluate its therapeutic potential in cervical cancer
SIGLEC15 is a novel immunosuppressive transmembrane protein promotes cancer development. However, the role of SIGLEC15 in cervical cancer remains poorly understood until now. In this study, we identified that the expression of SIGLEC15 was significantly increased in cervical cancer cells and tissues, and it was closely related to the metastasis of cervical cancer (70.6 %). Several anti-SIGLEC15 mAbs (named 6E3, 2B7, 3G2, 4F6, 4C4, 2F6) were prepared and identified, the titers were from 1:64000 to 1:128000. The results of IFA, IPMA, and IHC revealed that 6E3 (exhibiting a Kaff of 281,200,000 L/mol) had the capability to bind to cervical cancer cells and tissues. Then, the quantum dot nanoparticles probe (QDs-6E3) was synthesized and optimized. Moreover, the QDs based Immunochromatography Assay (QDs-ICA) method for the detection of SIGLEC15 was developed. After several evaluations, SIGLEC15 proved detectable at concentrations as low as 3 ng/mL within 4 min, demonstrating no cross-reactivity. The QDs-ICA exhibited a specificity of 96.7 % and a sensitivity of 96 %. The QDs-ICA for detecting SIGLEC15 is of great significance for the non-invasive screening of patients in clinical trials and the accurate evaluation of the therapeutic effect of this target in cervical cancer.