A NIR fluorescent probe based on tricyanofuran for the detection of β-galactosidase in living ovarian tumor cells and in vivo
β-Galactosidase (β-Gal) as an important glycoside hydrolase plays an important role in the early diagnosis of ovarian cancer. It is important to accurately and conveniently detect β-Gal in organisms. In this study, we developed a fluorescent probe TCF-GAL based on the tricyanofuran structure, which is linked to β-galactose through ether bonds for β-Gal detection. Probe TCF-GAL exhibited satisfactory selectivity and sensitivity toward β-Gal with the calculated LOD of 1.45 × 10