Examination of influence of microfluidic flow and antibody orientation on biosensor performance: A case study with CA125 electrochemical biosensor

Neelam Vishwakarma & Suman Singh et al. · 2025-12-31

Ovarian cancer has always posed a challenge to the healthcare industry due to a lack of early diagnosis methods, attributed to the absence of identifiable symptoms, thereby earning it the name "silent killer". Even though the traditional diagnostic methods provide some diagnostic capabilities, they still face several bottlenecks, highlighting the need to develop a robust, sensitive and accurate biosensor, specific to ovarian cancer biomarkers. In this study, an electrochemical platform with the microfluidic method of sample introduction was analyzed to detect cancer antigen 125 (CA125), a prominent biomarker in ovarian cancer patients. The platform was decorated with alkylamine-functionalized graphene sheets (A