E2F transcription factor-1 modulates expression of glutamine metabolic genes in mouse embryonic fibroblasts and uterine sarcoma cells

Katharina Huber & Lluis Fajas et al. · 2024-04-03

Metabolic reprogramming is considered as a hallmark of cancer and is clinically exploited as a novel target for therapy. The E2F transcription factor-1 (E2F1) regulates various cellular processes, including proliferative and metabolic pathways, and acts, depending on the cellular and molecular context, as an oncogene or tumor suppressor. The latter is evident by the observation that E2f1-knockout mice develop spontaneous tumors, including uterine sarcomas. This dual role warrants a detailed investigation of how E2F1 loss impacts metabolic pathways related to cancer progression. Our data indicate that E2F1 binds to the promoter of several glutamine metabolism-related genes. Interestingly, the expression of genes in the glutamine metabolic pathway were increased in mouse embryonic fibroblasts (MEFs) lacking E2F1. In addition, we confirm that E2f1
Authors
Katharina Huber, Albert Giralt, René Dreos, Helene Michenthaler, Sarah Geller, Valentin Barquissau, Dorian V. Ziegler, Daniele Tavernari, Hector Gallart-Ayala, Katarina Krajina, Katharina Jonas, Giovanni Ciriello, Julijana Ivanisevic, Andreas Prokesch, Martin Pichler, Lluis Fajas