Highly water-soluble AIEgen for α-amylase activity ultrasensitive detection and ovarian cancer rapid theranostic
Salivary α-amylase, as a digestive enzyme, is associated with variations in levels related to stress, diet, and certain medical conditions. Research indicates that it may have potential applications in assessing physiological and psychological stress responses. However, the clinical application of salivary amylase is still in the exploratory stage, lacking large-scale standardized testing methods. On the other hand, early theranostic of ovarian cancer remains a significant challenge, as traditional ultrasound examinations and tumor markers have limited effectiveness and insufficient specificity. Overall, both fields continue to evolve under clinical application and research drive, but further validation and improvement are still needed. In this study, we designed and synthesized a highly water-soluble AIEgen TPAG with a galactose structure, which not only could be used for ultrasensitive detection of α-amylase activity in saliva, but also for rapid diagnosis and therapy of ovarian cancer. Specifically, the limit of detection of α-amylase activity detection curve constructed based on TPAG absorbance changes was 0.004749 U/mL, and the limit of quantitation was 0.01439 U/mL. Even compared with commercial kits, the detection error still did not exceed 5 %, which further demonstrates the high reliability of this method. This method provides an effective tool for evaluating human sensory experience through the fluctuation of salivary α-amylase activity. Meanwhile, due to TPAG could be rapidly identified and taken up significantly by overexpressed β-galactosidase receptor in ovarian cancer, which providing a new tool for rapid theranostic of ovarian cancer. Salivary α-amylase detection and ovarian cancer screening are two important areas in current medical research. This innovative technology not only provides a fast and convenient detection method for monitoring salivary amylase levels, but also helps evaluate individuals' physiological and psychological stress states to promote the development of high-quality tobacco. It can also improve the early detection and precision therapy of ovarian cancer and make up for the limitations of traditional detection methods.