PAM-free activation of CRISPR/Cas12a via semi-nested asymmetric RPA: highly specific detection of HPV16 dsDNA

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Tao Gu; Xuheng Li; Changjun Hou

doi:10.1039/d5an00491h

PAM-free activation of CRISPR/Cas12a via semi-nested asymmetric RPA: highly specific detection of HPV16 dsDNA

Tao Gu & Changjun Hou et al. · 2025-08-18

SNA-RPA-CRISPR/Cas12a overcomes PAM limitations by converting dsDNA into ssDNA via semi-nested asymmetric amplification, enhancing HPV16 detection sensitivity and specificity by leveraging Cas12a's trans-cleavage activity of fluorescent reporters.

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The Analyst

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A semi-nested asymmetric recombinase polymerase amplification (SNA-RPA) method combined with CRISPR/Cas12a for the detection of HPV16 dsDNA, offering a promising solution for precise and reliable HPV diagnostics and cervical cancer screening.

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PAM-free activation of CRISPR/Cas12a via semi-nested asymmetric RPA: highly specific detection of HPV16 dsDNA

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