Ultrasensitive amplification-free detection of circulating miRNA via droplet-based processing of SERS tag–miRNA–magnetic nanoparticle sandwich nanocomplexes on a paper-based electrowetting-on-dielectric platform
Rapid (∼5 min) pretreatment and concentration of the MNP–miRNA–NAEB complex are accomplished using a pEWOD device. This facilitates sub-femtomolar SERS detection for analyzing microRNA in clinical samples of ovarian cancer.