Phosphoproteins Identification on Stretchable Photonic Crystal for Cervical Cancer Assessment
Cervical cancer is one of the diseases that threaten women's health. Early screening and assessment are crucial for disease intervention and treatment. However, existing diagnostic technologies generally have problems such as invasive, high cost, and complex operation, which limit their widespread application in clinical early screening. Therefore, there is an urgent need to develop a rapid, low-cost, minimally invasive, and highly sensitive method for cervical cancer assessment. Protein phosphorylation levels show specific dynamic changes at different stages of cervical cancer development. Herein, we report a phosphoprotein identification method based on a stretchable photonic crystal. Three fluorescent probes were loaded onto the stretchable photonic crystal that constructed a cross-response sensor. The photonic bandgap was dynamically regulated by mechanical stretching to match with the emission wavelengths of the multiple probes, thereby enhancing fluorescence and amplifying signal differences. Without the need for additional probe synthesis and labeling, our strategy can accurately pinpoint the stage of cervical cancer development based on serum samples, with an accuracy rate of up to 94%. This study provides a simple, efficient, and clinically promising detection method for the assessment of cervical cancer.
Journal
Analytical ChemistryTL;DR
Authors
Funding
National Key Research and Development Program of China Grant 2016YFC1100502National Key Research and Development Program of China Grant 2021YFA1200400Special Project for Research and Development in Key areas of Guangdong Province Grant 2020B010190003National Natural Science Foundation of China Grant 22090050National Natural Science Foundation of China Grant 22176180National Natural Science Foundation of China Grant 22474049National Natural Science Foundation of China Grant 52003103Key Laboratory of Bioinspired Materials and Interfacial Science Funding