Skin Research and Technology

RETRACTED: Preliminary Evaluation of the Value of a Small‐Molecule Probe Targeting DNMT1 in Detecting the Methylation of PAX1 in Cervical Cancer

ABSTRACT Background To investigate the screening value of a small‐molecule probe to assess the methylation of PAX1 in cervical cancer. Materials and Methods The diagnostic threshold of the grayscale values for cervical lesions was assessed by plotting the Receiver Operating Characteristic (ROC) curve of subjects. Grayscale values were significantly different among the four groups ( p  < 0.05). Compared with the LSIL and cervicitis groups, a considerably higher grayscale value was found in the CA and high‐grade squamous intraepithelial lesion (HSIL) groups (both p  < 0.05). Results The differential ROC curves of the grayscale values showed that the diagnostic Area Under Curve of the probe for cervicitis and low‐grade squamous intraepithelial lesion (LSIL) was 0.8724 (95% CI = 0.7762–0.9685, p  < 0.0001), for cervicitis and CA was 1.0000 ( p  < 0.0001), for the LSIL and HSIL was 0.5484 (95% CI = 0.3826–0.7142, p  = 0.5755), and for the LSIL and CA was 0.7724 (95% CI = 0.6016–0.9432, p = 0.0138). Conclusion The small molecular probe has certain application value in differentiating the type of cervical lesions and has better efficacy in distinguishing cervical inflammatory and precancerous lesions from carcinogenesis, but less efficacy in determining the type of precancerous lesions.

RETRACTED: Diagnostic value for methylation in cervical cancer based on a small‐molecule fluorescent probe targeting DNMT1

Abstract Background Value analysis of a small‐molecule fluorescent probe for methylation detection in different cervical lesions. Materials and Methods (1) The grayscale values of distinct lesion tissues were remarkably distinct among the four groups ( p  < 0.05). The comparison of the grayscale value between the two groups showed that the CA group noticeably exceeded the LSIL and cervicitis groups, and the HSIL group was apparently higher than the LSIL and cervicitis groups ( p  < 0.05); (2) The mean grayscale values of the enrolled subjects were calculated with 55.21 as the midline, with >55.21 as positive and ≤55.21 as negative. Results The results showed that the positive rate of the cervicitis group was 0.00%, the LSIL group 67.74%, the HSIL group 83.33%, and the CA group 100.00%. The results among the four groups were notably distinct ( p <  0.05); (3) The comparison among DAPI, probe, bright, and merged images of cervicitis, LSIL, HSIL, and CA indicated that different cervical lesions were with quite various stains. Conclusion The grayscale value, positive rate, and stained picture of distinct cervical lesions were remarkably different. The small‐molecule fluorescent probe has a good value in differentiating cervical lesions and can be considered for popularization and application.