Journal of Microbiology and Biotechnology

Development of Enzymatic Recombinase Amplification Assays for the Rapid Visual Detection of HPV16/18

Human papillomavirus (HPV) types 16 and 18 are the major causes of cervical lesions and are associated with 71% of cervical cancer cases globally. However, public health infrastructures to support cervical cancer screening may be unavailable to women in low-resource areas. Therefore, sensitive, convenient, and cost-efficient diagnostic methods are required for the detection of HPV16/18. Here, we designed two novel methods, real-time ERA and ERA-LFD, based on enzymatic recombinase amplification (ERA) for quick point-of-care identification of the HPV E6/E7 genes. The entire detection process could be completed within 25 min at a constant low temperature (35-43°C), and the results of the combined methods could be present as the amplification curves or the bands presented on dipsticks and directly interpreted with the naked eye. The ERA assays evaluated using standard plasmids carrying the E6/E7 genes and clinical samples exhibited excellent specificity, as no cross-reaction with other common HPV types was observed. The detection limits of our ERA assays were 10

Pine Needle Extract Applicable to Topical Treatment for the Prevention of Human Papillomavirus Infection

Most cervical cancers are associated with high-risk human papillomavirus (HPV) infection. Currently, cervical cancer treatment entails surgical removal of the lesion, but treatment of infection and preventing tissue damage are issues that still remain to be addressed. Herbal medicine and biological studies have focused on developing antiviral drugs from natural sources. In this study, we analyzed the potential antiviral effects of

A Comprehensive Review of Naringenin, a Promising Phytochemical with Therapeutic Potential

Chelidonium majus Induces Apoptosis of Human Ovarian Cancer Cells via ATF3-Mediated Regulation of Foxo3a by Tip60

Forkhead transcription factor 3a (Foxo3a) is believed to be a tumor suppressor as its inactivation leads to cell transformation and tumor development. However, further investigation is required regarding the involvement of the activating transcription factor 3 (ATF3)-mediated Tat-interactive protein 60 (Tip60)/Foxo3a pathway in cancer cell apoptosis. This study demonstrated that

Distinct Microbial Signatures along the Female Reproductive Tract in Endometrial Cancer Patients

Emerging evidence suggests that microbiota dysbiosis plays a critical role in the pathogenesis of endometrial cancer (EC), a leading cause of cancer-related deaths in women globally. However, few studies have simultaneously examined both the upper and lower genital tract microbiota in such individuals. In this study, we investigated alterations in microbiota composition across different parts of the female genital tract in a Chinese cohort of EC patients. Samples from 59 individuals (22 endometrial cancer patients; 8 endometrial hyperplasia patients, and 29 benign controls) were collected. In addition, a total of 58 vaginal swabs, 39 fallopian swabs, 16 peritoneal fluid samples, 36 urine swabs, and 34 endometrium samples were finally recruited. The composition of bacterial communities was determined by 16S ribosomal RNA Miseq sequencing. Specific taxa were significantly enriched in the EC group, including