Cellular and Molecular Biology

Clinical Significance of Detection of Human Papilloma Virus DNA and E6/E7 mRNA for Cervical Cancer Patients

This study aimed to explore the clinical significance of detection of human papillomavirus (HPV) DNA and E6/E7 mRNA for cervical cancer patients. For this purpose, a total of 300 patients with cervical lesions who performed the colposcopy examination for the cervix between January 2018 and January 2020 were divided into three groups according to the level of cervical intraepithelial neoplasia (CIN): Low-level group (Group A, n = 101), high-level group (Group B, n = 149), cervical cancer group (Group C, n = 50) and gynecological inflammation group (Group D, n = 60). Tissue samples were collected from subjects above to perform the immunohistochemistry for E6/E7 protein and detection of HPV DNA and HPV E6/E7 mRNA. The results showed that HPV DNA copies and the positive rates of protein, DNA and mRNA of HPV E6/E7 in Groups A, B and C were significantly higher than those in Group D (all P<0.05), while no significant difference was identified in the comparison of the positive rate and copies of DNA among Group A, B and C (P> 0.05); in the Group A, B and C, patients had a higher positive rate of E6/E7 mRNA than those in the Group D (P<0.05), while in the Group B and C, the positive rate and copies of mRNA of HPV E6/E7 and HPV E6/E7 proteins were all higher than those in the Group A (P<0.05). In addition, the specificity of HPV E6/E7 mRNA was inferior to HPV DNA in the detection of the low-level intraepithelial neoplasia, but it performed better in the detection of the high-level intraepithelial neoplasia and cervical cancer. In general, HPV E6/E7 mRNA shows promising value in the detection of the development and progression of cervical cancer.

The effect of supportive and educational nursing care on quality of life and HE4 gene expression in patients with ovarian cancer

Ovarian cancer is one of the most common malignancies in women and is also the fifth leading cause of cancer death in women worldwide. In recent years, the survival rate of patients with this disease has been long, and at the same time, more emphasis is on their quality of life. Therefore, the present study was conducted to investigate the effect of supportive and educational care of nurses on the life quality of patients with ovarian cancer. The expression of the HE4 gene was also evaluated as a diagnostic marker of ovarian cancer to assess the role of supportive and educational care of nurses in improving the physical health of these patients. In this study, which was a quasi-experimental study, 45 patients with ovarian cancer participated. The instrument was demographic information and quality of life questionnaires related to Beckman Institute, which were completed in two stages before and after patients' training and support sessions. HE4 gene expression was also assessed by Real-time PCR technique. Finally, the obtained data were analyzed using SPSS software and statistical tests. Based on the results, the mean score of quality of life before the intervention was 51.73 ± 13.91, and after the intervention was 60.46 ± 13.80 (P <0.001). Also, in all four dimensions of quality of life, the mean score of individuals after the intervention increased compared to before the intervention, but this difference was recognized as significant in only two dimensions of physical and mental health (P <0.001). The results of HE4 gene expression also showed that supportive and educational care of nurses had a significant effect on the expression of this gene. Therefore, this study confirmed the positive effect of educational and supportive programs in improving the quality of life of patients with ovarian cancer. In general, the design and implementation of such programs are proposed more widely and based on patients' educational and supportive needs.

TMCO1 regulates cell proliferation, metastasis and EMT signaling through CALR, promoting ovarian cancer progression and cisplatin resistance

Influences of total alkaloids in Caulis Mahoniae on proliferation and apoptosis of cervical cancer cells and the caspase-3 expression

Clinical significance of B7-H4 expression in ovarian cancer: a meta-analysis of proportions and time-to-event survival outcomes

Identification and characterization of differentially expressed genes in cervical cancer: insights from transcriptomic analysis

Melatonin Sensitizes OVCAR-3 Cells to Cisplatin through Suppression of PI3K/Akt Pathway

This study examined the effect of melatonin on oxidative stress, expression of pro-apoptotic protein, anti-apoptotic proteins, and the activity of the PI3K/Akt signaling pathway in the human ovarian cancer cell line (OVCAR3). OVCAR3 cells were treated with cisplatin, melatonin, cisplatin + melatonin, and siRNA Akt. Reactive oxygen species levels were assessed. The expression of the proteins was determined by Western blot. Melatonin administration significantly increased intracellular ROS generation, the cleavage of caspase 3 and decreased phosphorylation of Akt. Combination therapy of cisplatin and melatonin increases apoptosis in the OVCAR-3 cells by inhibiting of PI3K/Akt signaling pathway and exacerbating oxidative stress.

Expression Level of Keratin 7 in Epithelial Ovarian Cancer and Malignant Metastasis of Benign Epithelial Ovarian Tumors

It was to investigate the diagnostic value of keratin 7 (KRT7) in malignant metastasis of epithelial ovarian cancer and benign epithelial ovarian tumors. From January 2018 to January 2019, 30 fresh tissues of benign epithelial ovarian tumors, 30 fresh tissues of borderline tumors, 30 fresh tissues of metastatic ovarian were collected in The First Affiliated Hospital of Fujian Medical University, and 30 fresh tissues of normal ovarian tissues were collected as the control group. Federation of gynecology and obstetrics (FIGO) staging criteria: 25 cases of stage I, 26 cases of stage II, 16 cases of stage III, and 23 cases of stage IV. The relative expression of KRT7 was detected by real-time fluorescence quantitative PCR, and the relationship between KRT7 expression and epithelial ovarian cancer grading was analyzed. The results showed that the positive expression rate of KRT7 was 12.1% in normal ovarian tissues, 28.4% in benign epithelial ovarian tumors, 53.5% in borderline tumors, and 24.2% in metastatic ovarian cancer. With the increase of tumor stage malignancy, the relative expression of KRT7 decreased significantly, but there was no significant difference between stage I and stage II, stage III and stage IV (P &gt; 0.05). The difference between stage I and stage III, and stage IV was significant (P &lt; 0.05). Patients with epithelial ovarian cancer had a significant difference compared with the control group (P &lt; 0.05). In summary, compared with the control group, the expression of KRT7 in patients with benign epithelial ovarian tumors and borderline tumors was significantly decreased. The expression level of KRT7 in benign epithelial ovarian tumors was lower than that in borderline tumors. The expression of KRT7 was related to the occurrence, development, and deterioration of ovarian cancer, which provided a basis for targeted therapy of tumors.

Regulation of Transcription Factor YAP-TEAD by Non-coding RNA LINC00857 and the Inhibitory Effects on Ovarian Cancer Cell Proliferation

This study was aimed to explore the expression and mechanism of the transcription factor YAP-TEAD in the Hippo signaling pathway under the regulation of non-coding Ribonucleic Acid (RNA) LINC00857 in the proliferation of ovarian cancer cells, so as to provide a scientific research basis for clinical diagnosis and treatment of ovarian cancer. In the study, the ovarian cancer cell lines (BT 549) were rolled into a control group (normal culture-defined as BT549/NC) and a response group (transfected with non-coding RNA LINC00857 cultured cells-defined as BT 549YAP cells). The expression and proliferation ability of the transcription factor YAP-TEAD in the two groups of cancer cells were analyzed and compared. The results showed that the YAP-TEAD expression rate was the highest in Bt549 cells; the YAP content grade (0.18) in BT 549-YAP cells was lower than BT 549/NC (0.2) after transfection (P&lt; 0.05); and the apoptotic rate of the response group (80%) was higher than that of the control group (25%) after the intervention. With the extension of culture time, the expression of CCN1 mRNA decreased (P&lt; 0.05), and CCN2 mRNA increased (P&lt; 0.05). After 12, 24, 36, and 48 hours, the apoptosis rate of the reaction group at different time points was higher than that of the control group (P&lt; 0.01). When YAP-TEAD was down-regulated, the in vitro proliferation ability of BT 549- YAP cells was weakened compared with BT 549/NC and parental cells. It was concluded that the noncoding RNA LINC00857 can target the transcription factor YAP-TEAD in the Hippo signaling pathway to decrease its expression, thus inhibiting the proliferation, migration, and invasion of cancer cells, and promoting cell apoptosis.

MiR-145 on the Proliferation of Ovarian Cancer Cells by Regulating the Expression of MMP-2/MMP-9

This study aimed to consider the effect of miR-145 on the proliferation of OC cells by regulating the expression of MMP-2/MMP-9. In this experiment, the miR-145 target gene with restriction enzyme cut sites was amplified from the four groups of rat genomes, and it was ligated to the pIRES plasmid to form a recombinant plasmid and identified. The miR-145 recombinant plasmid and the empty plasmid into OC cells were transfected, and qRT-PCR was used to detect the expression of miR-145 in the transfected OC cells. Experimental data showed that there are a total of 60 healthy male SD rats, randomly divided into 4 groups, namely the normal saline control group (group A), the model group ( group B), treatment group with 30 mg miR-145 (group C), and treatment group with 100mg miR -145 (group D). The experimental results showed that compared with group A, the expression of MMP-2 protein in rats in groups B, C, and D all increased to varying degrees on 7 days, 14 days, and 28 days, reaching the highest at 14 days. This study shows that miRNA-145 is under-expressed in OC cell lines, and miR-145 can significantly inhibit the proliferation and invasion of ovarian cells by regulating the expression of MMP-2/MMP-9.

The effect of Chaihu-shugan-san on cytotoxicity induction and PDGF gene expression in cervical cancer cell line HeLa in the presence of paclitaxel +cisplatin

Chaihu-shugan-san, as a traditional Chinese herbal formula, is composed of seven different herbs. This medicine can treat cancer due to its antioxidant compounds. In this study, the effect of Chaihu-shugan-san was considered on cytotoxicity induction and PDGF gene expression in cervical cancer cell line HeLa at different concentrations and at different times, by the MTT method. Paclitaxel + cisplatin were used as a control in this study. The expression of the PDGF gene was quantitatively evaluated in treated cells by real-time PCR, and a generalized linear model was used to evaluate the effect of the medicine, and Duncan's multiple range tests were used to evaluate the data. The results of the MTT test showed that Chaihu-shugan-san had antitumor properties in different concentrations, but there was a significant difference between this medicine and paclitaxel +cisplatin. Also, examination of gene expression showed that this medicine reduced the expression of the PDGF gene in the HeLa cancer cell line (P ? 0.04). Therefore, Chaihu-shugan-san could be suggested as an effective factor in preventing the growth of cervical cancer cells and controlling angiogenic factors that play an important role in the metastasis of cancerous tumors.

An integrated model of CDCA5 and FOXM1 expression combined with a residual disease that predicts prognosis in ovarian cancer patients

Chelerythrine induces apoptosis and ferroptosis through Nrf2 in ovarian cancer cells

Increased risk of ovarian and breast malignancies in women with polycystic ovary syndrome: a review article

Mutation analysis of the TERT gene in ovarian cancer patients of the Turkish population by next generation sequencing method

N6-Methyladenosine modified circ-NAB1 modulates cell cycle and epithelialmesenchymal transition via CDKN3 in endometrial cancer

Research progress on the mechanism of action of Salvia miltiorrhiza and its components in the treatment of endometrial carcinoma

Next-generation sequencing and immunohistochemistry approaches for microsatellite instability detection in endometrial cancer

The disulfidptosis-related lncRNAs can predict survival and immunotherapy response accurately in endometrial carcinoma

Identification of core genes shared by endometrial cancer and ovarian cancer using an integrated approach

Expression of miR-128-3p, miR-193a-3p and miR-193a-5p in endometrial cancer tissues and their relationship with clinicopathological parameters

Comparison between Pelvic IMRT and 3D-CRT in Combination with Chemotherapy via Nrf2 Expression on the High-Risk Endometrial Cancer

This study aimed to explore the clinical efficacy of pelvic intensity-modulated radiation therapy (IMRT) and 3-dimensional conformal radiotherapy (3D-CRT) in combination with chemotherapy on high-risk endometrial cancer. The effect of these methods is evaluated via Nuclear factor (erythroid-derived 2)-like 2 (Nrf2) expression, the levels of chitinase protein 40 (YKL-40), human epididymis protein 4 (HE4), and prolactin (PRL) in serum. For this purpose, between August 2014 and July 2017, 114 endometrial cancer patients admitted to this hospital for treatment were randomized into the observation group (n=60) and control group (n=54). Following the surgery, patients in these two groups received the chemotherapy of taxol and carboplatin (TC). Based on the chemotherapy, patients in the observation underwent the IMRT, while those in the control group adopted the 3D-CRT. The Nrf2 expression was performed based on the Real-time PCR technique. The incidence rate of adverse reactions was a 3-year recurrence rate and mortality rate. Results showed that after treatment, levels of YKL-40, HE4, and PRL in the serum of patients in two groups decreased compared to those before treatment (all P &lt; 0.05). In comparison, the difference between the two groups showed no statistical significance (P &gt; 0.05). The evaluation of Nrf2 transcription factor expression showed significant differences started in comparisons of the Nrf2 Expression between two groups (P &gt; 0.05), and this enhancement was significant in the control group after treatment. Comparison of the incidence rates of the bone marrow suppression during treatment showed no significant difference (P &gt; 0.05). However, the incidence rates of radiation enteritis and radio-cystitis in the observation group were much lower than those in the control group (P &lt; 0.05). During the follow-up, there were five patients in the control group and 7 in the observation group losing to the follow-up, and among the remaining subjects, no significant difference was identified in the comparison of the recurrence rate or mortality rate between the two groups (all P &gt; 0.05). In general, Pelvic IMRT in combination with chemotherapy is a promising and safe candidate for high-risk endometrial cancer with mild radiation injury; besides, YKL-40, HE4, and PRL are the effective indicator for the prediction of efficacy in chemotherapy for endometrial cancer.

Early detection of cervical tumor cell of origin through Oncogene-induced senescent HPV-positive cells

MiR-4319 targets tuftelin 1 to reduce malignancy of cervical cancer cells

The effects of laparoscopic and laparotomy extensive hysterectomy on the safety of ureterovaginal fistula infection in patients with cervical cancer

MiR-188-5p inhibits cell proliferation and migration in ovarian cancer via competing for CCND2 with ELAVL1

Phytochemical investigation and effective therapeutic potential of plants extracts against breast and ovarian cancer cell lines: compounds from zizyphus mauritiana and triticum aestivum

ancer is the leading cause of death, accounting for approximately one out of six people dying with this disease worldwide. Among all, the breast and ovarian cancers are top-ranked causes of women mortalities compared to other disorders. Although, there is advancement in technologies, but still, there are unresolved concerns to overcome the global disease burden. Currently, plants are being explored as a natural remedy to cure disorders. This research was planned to explore phytochemicals in methanolic extracts of Zizyphus mauritiana and Triticum aestivum, and their pharmacological activities were studied through Agrobacterium tumefaciens bacteria, in vitro breast cancer cell line and ovarian cancer cell line to find out novel candidates in disease control and prevention. Eleven different types of bioactive compounds were analysed in the tested extracts. The highest crude extracts percentage (75±0.02) was observed with Z. mauritiana. The extracts showed promising cell growth inhibition and tumor initiation inhibition in potato disc assay. MTT assay and Incucytes imaging analysis revealed that Z. mauritiana extract had a higher anticancer potential with 40 ± 0.92 cell viability against breast cancer cells (SKBR3) and 45 ±0.29 against ovarian cancer cells (SKOV3). In conclusion, these extracts could be used as chemotherapeutics owing to their cheapness, and easy availability. While detailed study is required for further purification and characterization of bioactives/target compounds and in-vivo activity confirmations.

Evaluation of risk factors and effective factors in determining the prognosis of ovarian cancer

eTranscriptional factor CCAAT enhancer binding protein beta inhibits epithelial-mesenchymal transition in cervical cancer via regulating attractin-like 1

Effect of MiR-10b on cervical cancer rats through mTOR/P70S6K signaling pathway

Expression and clinical significance of PD-1 in UCEC and its impact on tumor

HDAC1 suppresses radiotherapy sensitivity in cervical cancer via regulating HIF-1α/VEGF signaling pathway

MiR-1299 is regulated by KCNQ1OT1 and inhibits cervical cancer progression

MiR-214-3p suppresses cervical cancer cell metastasis by downregulating THBS2

The prognostic, immunological and single-cell features of m6A molecules in cervical cancer

Comparative evaluation of cytotoxic and anti-metastatic function of microbial chondroitin sulfate and animal-originated commercial chondroitin sulfate in cancer cells

The expression and clinical value of LRP11, FUBP1 and TET1 in cervical cancer

DUSP1 regulates the JAK2/STAT3 signaling pathway through targeting miR-21 in cervical cancer cells

Association of microRNA-10b expression and P16 with pathological features in various stages of cervical precancerous lesions

Overview of the signaling pathways involved in metastasis: An intriguing story-tale of the metastatic journey of ovarian cancer cells

Wealth of information has revolutionized our understanding related to the genetics and functional genomics of this heterogeneous disease. Keeping in view the heterogeneity of ovarian cancer, long-term survival might be achieved by translation of recently emerging mechanistic insights at the cellular and molecular levels to personalize individual strategies for treatment and to identify biomarkers for early detection. Importantly, the motility and invasive properties of ovarian cancer cells are driven by a repertoire of signaling cascades, many components of which have been experimentally verified as therapeutic targets in preclinical models as well as in clinical trials. Scientific evidence garnered over decades of research has deconvoluted the highly intricate intertwined network of intracellular signaling pathways which played fundamental role in carcinogenesis and metastasis. In this review we have provided a compendium of myriad of signaling cascades which have been documented to play critical role in the progression and metastasis of ovarian cancer. We have partitioned this multi-component review into different sections to individually discuss and summarize the roles of TGF/SMAD, JAK/STAT, Wnt/?-Catenin, NOTCH, SHH/GLI, mTORC1/mTORC2, VEGFR and Hippo/YAP pathways in ovarian cancer metastasis.

Betulin terpenoid targets OVCAR-3 human ovarian carcinoma cells by inducing mitochondrial mediated apoptosis, G2/M phase cell cycle arrest, inhibition of cell migration and invasion and modulating mTOR/PI3K/AKT signalling pathway

The main purpose of the current research work was to study in vitro anticancer effects of betulin in OVCAR-3 human ovarian carcinoma cells along with examining its effects on cellular apoptosis, cell cycle phase distribution, cell migration and invasion and mTOR/PI3K/AKT signalling pathway. The cell proliferation of OVCAR-3 cells at various doses of the drug was studied by CCK8 cell viability assay. Effects on cell apoptosis were studied by fluorescence microscopy and western blot. Effects on cell cycle were evaluated by flow cytometry and western blot. Transwell assays were used to study effects on cell migration and invasion. The results indicated that betulin led to significant reduction of OVCAR-3 cell viability in a dose-dependent as well as time dependent manner. Betulin also led to reduction in cell colonies. The anticancer effects of betulin were due to the induction of apoptosis which was seen by increased apoptotic cells with yellow and orange fluorescence. Betulin prompted mitochondrial apoptosis which was also associated with alteration in the apoptosis-related protein expression (Bax, Bad and Bcl-2 and Bcl-xL). The molecule also led to G2/M phase cell cycle arrest on OVACR-3 ovarian carcinoma cells. It was also observed that betulin could inhibit the migration and invasion of the ovarian cancer cells in a concentration-dependent manner. Betulin molecule also resulted in blocking of mTOR/PI3K/AKT signalling pathway.  In conclusion, this study clearly indicates the anticancer effects of betulin natural product in OVCAR-3 human ovarian cancer cells are mediated via apoptosis induction, G2/M phase cell cycle arrest, cell migration and invasion inhibition and targeting of mTOR/PI3K/AKT signalling pathway.

Antiproliferative effects of EGFR inhibitor Cetuximab and parp inhibitor combination on non-small cell lung cancer cell line A549 and cervical cancer cell line HeLa

Investigation on antimicrobial, antioxidant, and anti-cancerous activity of Agaricus bisporus derived β-Glucan against cervical cancer cell line

Value of TCT combined with HPV and CA125 in early cervical cancer screening in a medical examination population

Analysis of Mechanism of Action of Cuprous Oxide Nanoparticles in Treatment of Cervical Cancer under Real-time Ultrasound Elastography

It aimed to explore the adoption value of cuprous oxide nanoparticles (Cu2O NPs) in the clinical treatment of cervical cancer under the evaluation of real-time ultrasound elastography. In this experiment, the solution of Cu2O NPs was synthesized and used in 90 selected mouse models of cervical cancer. It was found that Cu2O NPs can significantly control the reproduction of various types of cervical cancer cells, effectively stopping the cycle of cervical cancer cell lines in the G1/G0 phase. In addition, the tumor weight of 0.25g in the Cu2O NPs group was notably lighter than that of 1.1g in the control group. The weight change of the mouse was 21g compared with 15g of the cis-dichlorodiamine platinum (CDDP) group, and it was proved that Cu2O NPs were less cytotoxic to the body and have few side effects. Moreover, real-time ultrasound elastography showed that there were 26 cases with a tumor elasticity score no less than 2 in the Cu2O NPs group, accounting for 87%, which was better than that of the CDDP group (17 cases, 57%), and the difference was substantial (P<0.05). The shear wave velocity of Cu2O NPs (1.46±0.48 m/s) was also lower than that (1.73±0.62 m/s) of the CDDP group, which suggested that the tumor body hardness of mice in the Cu2O NPs group was lower, and the difference was considerable (P<0.05). In short, Cu2O NPs had good functions such as inhibiting the proliferation and spread of tumor cells and blocking the cell cycle. Moreover, the toxic and side effects of the drug were slight, and it was an ideal new type of treatment for cervical cancer.

MiR-494 Inhibits the Proliferation, Migration and Invasion of Cervical Cancer Cells by Regulating LETMD1

LETMD1 is a differentially expressed gene selected by scientists from cervical cancer (CC) tissues by RT-PCR technology. It has been confirmed that LETMD1 is overexpressed in many human malignant tumors, so it can be used as an early diagnostic marker for malignant tumors and as a target for gene therapy. The purpose of this article is to further explore the effectiveness of miR-494 in inhibiting the proliferation, migration and invasion of CC cells by regulating LETMD1, selecting 40 cases of CC admitted to a hospital from June 2015 to September 2018 Patients, tumor tissue specimens were taken from the primary tumor tissue of CC, and normal tissues near the cutting edge were collected as controls. Normal tissues were confirmed by pathology after surgery that they were not invaded by cancer tissues. The results of the study showed that the expression level of miR-494 increased by 15%, and the prognostic survival rate after surgery increased by 20%, depending on gender, age, tumor size, and tumor site. After high expression of miR-494 in CC patients, the vascular invasion of CC cells was reduced by 33%, and distant metastasis was reduced by 11%, and the survival time of patients was significantly prolonged. After the expression of LETMD1, the proportion of cancer cells decreased by 5%, the proportion of macrophages increased by 2%, and the dendritic cells increased by 3%.

MiR-218 Affects the Invasion and Metastasis of Cervical Cancer Cells by Inhibiting the Expression of SFMBT1 and DCUNIDI

As the second most common gynecological tumor in the world, cervical cancer has always been a female killer. Some studies have shown that miR-218 can inhibit the invasion and migration of cervical cancer cells through two target genes, SFMBT1and DCUNIDI. On this basis, this paper presents the study that miR-218 affects the invasion and migration of cervical cancer cells by inhibiting the expression of target genes, SFMBT1 and DCUNIDI. In this paper, we used the control experiment to study. In the fluorescence experiment, we confirmed that SFMBT1 and DCUNIDI are indeed the downstream target genes of miR-218. After that, we down-regulated or up-regulated the expression of SFMBT1 and DCUNIDI in cervical cancer cells through specific small interfering RNA and overexpression plasmids, so we carried out cell migration and cell invasion experiments. The results of the cell invasion experiment showed that the average value of cell invasion ability of the miR-218 group was 286 and 218, that of SFMBT1-siRNA1 was 264, that of DCUNIDI-siRNA1 was 179, that of SFMBT1-siRNA 2 was 245, that of DCUNIDI-siRNA2 was 196, and that of SFMBT1 + miR-218 was 401. The average cell invasiveness of DCUNIDI + miR-218 was 672.

The regulating role of miR-494 on HCCR1 in cervical cancer cells

This study focused on miR-494 inhibiting the proliferation, migration and invasion of cervical cancer cells by regulating HCCR1. During the study, 30 pairs of primary cervical cancer tissues and adjacent tissues diagnosed by pathology were collected, and then placed in a cryopreservation tube, immediately placed in liquid nitrogen to freeze, and then moved to a -80 °C refrigerator for storage until use. They were dipped with crystal violet staining solution for 20 minutes, washed 3 times with PBS, and dried at room temperature. The polycarbonate film on the chamber was gently cut, placed on a glass slide, and covered with neutral resin. The number of cells in 5 random fields was counted under a microscope, and the differences between the groups were compared. The qRT-PCR results showed that compared with the normal cervical tissue cell line HCCR1, the expression levels of miR-494 in cervical cancer cell lines HCCR1 and C-33A were significantly reduced. Compared with the transfection of mimics NC, the level of HCCR1mRNA and protein decreased significantly when transfected with miRNA-494mimics (P &lt;0.05); compared with the transfection of inhibitor NC, the level of HCCR1 mRNA and protein increased significantly when transfected with miRNA-494 inhibitor (P &lt;0.05).

M2 macrophages regulate KDM6B/PFKFB2 metabolic reprogramming of cervical squamous cell carcinoma through CXCL1