Zhiwei Qiao

The lncRNA SNHG15/miR‐18a‐5p axis promotes cell proliferation in ovarian cancer through activating Akt/mTOR signaling pathway

AbstractHere, we report the expression pattern, function and regulatory mechanism of SNHG15 together with miR‐18a‐5p micro RNA in ovarian cancer (OC) for the first time. We recruited 20 patients and took normal ovarian tissues and ovarian tumor tissues from them. We used cell culture, transfection, in vivo tumor xenograft assay, and multiple types of detection assays to investigate the expression and regulation of long noncoding RNA (lncRNA) SNHG15/miR‐18a‐5p in ovarian tissues and cells. Results: We found that the messenger RNA expression level of SNHG15 was significantly higher and miR‐18 was decreased in ovarian cancer tissues and in OC cells. Functional experiments showed that SNHG15 overexpression potentiated the migration and invasion of OC cells, while SNHG15 inhibition reduced the tumor proliferation, which was restored via overexpression of miR‐18a. SNHG15 was found to directly target and suppress the expression of miR‐18a. Our results illustrate the possible molecular mechanism of lncRNA SNHG15/miR‐18a‐5p functions in cell proliferation in OC. SNHG15/miR‐18a promoted the progression of OC cells via the protein kinase B/mammalian target of rapamycin signaling pathway.