Xuedong Tang

The Expression and Clinical Significance of C1orf106 in Low‐Grade Serous Ovarian Cancer

ABSTRACT Aim Low‐grade serous ovarian cancer (LGSOC) is a rare subtype of ovarian cancer with distinct biological behavior. This study aimed to identify new biomarkers with potential diagnostic and prognostic value for LGSOC. Methods Gene‐expression data were downloaded from the Gene Expression Omnibus (GEO). Differentially expressed genes (DEGs) were identified using R. Functional enrichment analyses were conducted to determine the biological functions and signaling pathways associated with DEGs. The mitogen‐activated protein kinase (MAPK) pathway‐related gene, chromosome 1 open reading frame 106 ( C1orf106 ), was selected as the target gene. Immunohistochemistry and quantitative real‐time polymerase chain reaction (qRT‐PCR) were performed to verify its expression. Associations between C1orf106 expression and the clinical features of patients were analyzed using the chi‐square ( χ 2 ) test. Prognostic significance was evaluated with survival analyses. Results A total of 3099 upregulated and 4968 downregulated genes were identified in LGSOC. Gene set enrichment analysis (GSEA) demonstrated significant alterations in KRAS signaling and metabolic pathways between LGSOC and healthy controls. Kyoto Encyclopedia of Genes and Genomes (KEGG) and Gene Ontology (GO) analyses revealed enrichment in immune response and MAPK pathway alterations. Immunohistochemistry and qRT‐PCR confirmed that C1orf106 expression in LGSOC tissues was significantly higher than in normal ovarian tissues. Clinically, high C1orf106 expression was associated with lower BMI (< 25 kg/m 2 ), the absence of visible residual disease, and improved progression‐free survival (PFS) and overall survival (OS) in univariate Cox and Kaplan–Meier analyses. Conclusions C1orf106 may serve as a promising marker for the diagnosis and prognosis of LGSOC.

Application of Single-Hole Laparoscopy and Sentinel Lymph Node Imaging in Early Endometrial Carcinoma in Special Population

Aim. To explore the clinical efficacy of single-hole laparoscopy combined with sentinel lymph node imaging in the treatment of early endometrial carcinoma in a special population. Method. A retrospective analysis was made on the clinicopathological data of 8 patients with early endometrial carcinoma who underwent extra fascial total hysterectomy plus double adnexal resection and pelvic sentinel lymphadenectomy by transumbilical single-hole laparoscopy in Jiaxing Maternal and Child Health Hospital from Apr. 2019 to Apr. 2021. Result. Single-hole laparoscopy and sentinel lymph node imaging were successfully performed in 8 patients with early endometrial carcinoma, and none of them was converted to porous or laparotomy. At the same time, all 8 patients have a high demand for body shape. All FIGO pathological grades were grade I before operation. Operation time is 160.87 ± 40.61   min , amount of bleeding is 68.75 ± 12.31   ml , the catheter was removed for 2 days, anal exhaust time is 30.13 ± 10.99   h , and postoperative hospital stay is 4.00 ± 1.07   d . There was no related organ injury during the operation, no case of blood transfusion, or case of poor wound healing. The evaluation of postoperative satisfaction was very satisfactory. Conclusion. The application of single-hole laparoscopy and sentinel lymph node imaging in the treatment of early endometrial carcinoma in the special population should be safe and feasible with high satisfaction.