Wei Sun

Tissue-, Blood-, and Urine-Based Proteomics Changes of Different Endometrial Receptivity Status in Patients after Fertility-Sparing Treatment of Atypical Endometrial Hyperplasia or Endometrial Cancer

Liquid biopsy noninvasively characterizes diseases by analyzing biomarker proteins in biofluids, which provide valuable insights into physiological and pathological processes. In this study, we conducted an analysis of the differences between atypical endometrial hyperplasia or endometrial cancer (AH/EC) patients after fertility-sparing treatment with different RNA-Seq-based endometrial receptivity test (rsERT) results ("receptive" versus "pre-receptive"), to investigate the proteomic connections among tissue, serum, and urine samples. Samples of endometrial tissue, serum, and urine from 40 rsERT "pre-receptive" and 10 rsERT "receptive" patients were analyzed for proteomic profiling. We integrated differentially expressed proteins from three sample types to investigate endometrial receptor (ER)-related molecular changes. Our findings indicated that both serum and urine proteomes can serve as indicators of functional changes in endometrial tissue. In serum, proteins associated with cholesterol metabolism, immune response, and coagulation exhibited a differential expression. In urine, proteins related to immune function and metabolic processes demonstrated varying levels of expression. The differentially expressed proteins in both serum and urine were associated with the immune response and metabolism. In conclusion, biofluids serve as a reflection of functional changes in endometrial tissue, which will facilitate a deeper understanding of endometrial receptivity and the discovery of potential clinical biomarkers.

A national survey on HPV vaccination status among 42,800 female physicians and nurses in China, 2021

Human papillomavirus (HPV) vaccination is the most effective method to prevent cervical cancer. This study aimed to investigate the status of HPV vaccination and associated factors among Chinese females. Between January and March 2021, we conducted a large national survey among female doctors and nurses in 181 public tertiary hospitals across all 31 provinces of China. In the survey, we asked three questions: "Have you ever received an HPV vaccination? If yes, what type and in which year?" We described and compared the proportion of vaccination coverage according to occupation, age, geographic region, education, marital status, among other factors. Among 42,800 participants, 6185 (14.45 %) reported receiving HPV vaccination. Physicians showed a slightly higher vaccination rate (2064/13,804; 14.95 %) than nurses (4121/28,996, 14.21 %). Factors significantly associated with higher vaccination rates included younger age, being never married or divorced, higher education, better self-reported health status, residing in the western region, working in Obstetrics and Gynecology or Surgery departments, working at cancer hospitals, and being nulliparous. Regarding vaccine types, the 4-valent vaccine accounted for 50.3 %, followed by the 9-valent (33.7 %), imported 2-valent (16.0 %). Understanding the characteristics of the likelihood of receiving HPV vaccination among female physicians and nurses in China indicates their awareness of the risk for cervical cancer, which could help us better develop primary prevention strategies.