Reza Safaralizadeh

Upregulation of PCAT1, PCAT2, and PCAT3 LncRNAs in cervical cancer patients and their diagnostic value

Cervical cancer is one of the most common cancers among women. Despite advances in vaccination and screening, barriers to early detection and effective treatment remain. lncRNAs play an important role in cancer progression and therapeutic responses. New lncRNAs such as PCAT1, PCAT2, and PCAT3 have shown oncogenic activities in multiple cancers, but their role in cervical cancer is not understood. This study investigated the expression patterns of PCAT1, PCAT2, and PCAT3 in cervical cancer to evaluate their use as diagnostic markers and therapeutic targets. The expression level of PCAT1, PCAT2, and PCAT3 was significantly (P value < 0.05) higher in tumor tissues.ROC analysis showed a moderate diagnostic biomarker value for PCAT1 (AUC 0.79, sensitivity 79%, specificity 69%). Spearman's analysis showed a positive correlation between PCAT1-PCAT2 and PCAT1-PCAT3. PCAT1, PCAT2, and PCAT3 lncRNAs significantly increase in cervical cancerous tissues and may play a role as novel oncogenes. Their use as clinical diagnostic markers requires further studies.

Alterations in the expression levels of long intergenic non-coding RNA APOC1P1-3 in cervical cancer tissue samples

lncRNAs play a crucial role in the carcinogenesis process. Thus, they have been recognized as the potential therapeutic and diagnostic biomarkers of cancers. This study assessed the alteration in the expression of APOC1P1-3 lncRNA in cancerous tissues compared to their adjacent non-tumorous tissues sampled from cervical cancer patients. one hundred fifteen pairs of cancerous and adjacent non-cancerous biopsy of cervical cancer specimens were collected. RNA isolation and cDNA synthesis were carried out. The qRT-PCR was used to assess the changes in the expression of APOC1P1-3 lncRNA. Moreover, the biomarker function of the lncRNA and the correlations between APOC1P1-3 and clinicopathological parameters were measured. The APOC1P1-3 expression was significantly increased in cervical cancer specimens as compared to adjacent non-tumorous specimens (