Investigator

Rekha Kumar

Senior Consultant/Emeritus Professor · Sri Shankara Cancer Hospital and Research Center, Histopathology

RKRekha Kumar
Papers(1)
Downregulation of Dec…
Collaborators(9)
Shahid HussainVarun RaghunathanAnnapurna VadapartyArindam NahaAruna KorlimarlaHari P. S.Jyothsna Konkada Mana…Purba SarkarRamray Bhat
Institutions(2)
Sri Shankara Cancer H…University of Muenster

Papers

Downregulation of Decorin in ovarian cancer cells and colonization microenvironment drives progression

Epithelial ovarian cancer is a gynecological disease in which transformed cells, upon dissemination into the peritoneum colonize locales such as omenta and form metastatic foci. Colonization is an emergent outcome of the interactions between the invading cancer cells and extracellular matrix (ECM) of the peritoneal serosa. Although ECM is known to be remodeled in cancer, the dynamics in ovarian cancer of a major class of ECM-remodeling factors: the proteoglycans remain understudied. Here, we focus on Decorin, a proteoglycan with binding activity to the principal stromal ECM protein Collagen I and investigate its regulation of ovarian cancer colonization. We observe that Decorin is depleted in cancer deposits within omenta of cancer patients. The spreading of suspended spheroids of the ovarian cancer line SK-OV-3 on engineered Collagen I scaffolds is impaired when the latter is polymerized in the presence of Decorin. Decorin-supplemented Collagen I shows poorer fibrillar organization, which has been associated with slower kinetics of cancer cell migration. To our surprise, Decorin was also found to be depleted in primary tumor cells as well as in ovarian cancer cell lines compared with their controls. Overexpression of wild type Decorin, but not its glycosaminoglycan (GAG)-removed mutant in cancer cells decreased mean spheroid size, invasion through Collagen I matrix, and migration on fibronectin matrix scaffolds. Our results suggest that downregulation of an extracellular inhibitor of colonization occurs both in the seed and soil components of the metastatic toolkit; in addition, the GAG chains of Decorin may be crucial to its carcinomatosis-inhibiting functions.

102Works
1Papers
9Collaborators
Ovarian NeoplasmsCell Line, TumorTumor Microenvironment

Positions

2019–

Senior Consultant/Emeritus Professor

Sri Shankara Cancer Hospital and Research Center · Histopathology

1987–

Professor and Head

Kidwai Memorial Institute of Oncology · Pathology

Education

1986

Bangalore Medical College · MD Pathology

1981

MBBS

Christian Medical College & Hospital · Christian Medical College