Piotr Lewitowicz

Endometrial Cancer in Aspect of Forkhead Box Protein Contribution

(1) Background: The present study aimed to investigate the influence of forkhead box (FOX) on endometrial cancer (EC) progression. For a better understanding, the driving mechanisms are vital to identifying correlations between genes and their regulators. (2) Methods: The study enrolled one hundred and three white female patients with confirmed EC. For the analysis, we used next-generation sequencing with the Hot Spot Cancer Panel provided by Illumina Inc., San Diego, CA, USA, and an immunohistochemical analysis of FOXA1, FOXP1, and estrogen receptors. (3) Results: FOXA1 silencing led to a worse outcome based on the correlation with FOXA1 (test log-rank p = 0.04220 and HR 2.66, p = 0.033). Moreover, FOX proteins were closely correlated with TP53 and KRAS mutation. (4) Conclusions: Our study confirmed previous reports about FOX box protein in the regulation of tumor growth. A remarkable observation about the unclear crosstalk with crucial genes, as TP53 and KRAS need deeper investigation.

Disseminated Peritoneal Leiomyomatosis of the Peritoneum Following Laparoscopic Supracervical Hysterectomy with Morcellation: A Case Report

BACKGROUND Disseminated peritoneal leiomyomatosis is a rare complication of laparoscopic procedures involving uterine morcellation. These fibroid-like lesions, which derive their blood supply from non-uterine sources, may develop after myomectomy, supracervical hysterectomy, or total laparoscopic hysterectomy - particularly when mechanical morcellation is used. Although the incidence is low, disseminated leiomyomatosis should be considered in the differential diagnosis of abdominal or pelvic pain in patients with a history of such surgeries. CASE REPORT A 40-year-old woman presented with intermittent lower abdominal pain. She had undergone a laparoscopic supracervical hysterectomy with salpingectomy 5 years earlier due to abnormal uterine bleeding, during which uncontained mechanical morcellation was performed. At present, transvaginal ultrasound revealed a solid mass adjacent to the bladder with features suggestive of a leiomyoma. Magnetic resonance imaging (MRI) showed multiple lesions, including a polycyclic mass located above the bladder and additional foci near the sigmoid colon and the bladder dome. Laparoscopy confirmed the presence of leiomyomas on the anterior abdominal wall, sigmoid colon adventitia, and bladder dome. All lesions were excised using laparoscopic techniques, and the postoperative course was uneventful. Histopathological examination confirmed leiomyomas with positive estrogen and progesterone receptor expression. CONCLUSIONS This case underscores the importance of considering disseminated peritoneal leiomyomatosis in the differential diagnosis of abdominal or pelvic pain in patients with a history of laparoscopic hysterectomy with morcellation. It also emphasizes that this condition can mimic malignancy on imaging studies and highlights the essential role of histopathological examination in diagnosing this benign disease.

Sexual Behavior and the Awareness Level of Common Risk Factors for the Development of Cervical, Anogenital and Oropharyngeal Cancer among Women Subjected to HR HPV DNA-Testing

The human papillomavirus (HPV) is a significant health problem that increases the risk of developing precancerous lesions, cancer of the anogenital area, as well as mouth and throat cancers. The aim of the study was to analyze the awareness level of common risk factors and the preferred sexual behavior of women aged 18–68, who underwent a molecular evaluation of common HR-HPV from material collected from the cervix. The study was conducted at the Jan Kochanowski University in Kielce, Collegium Medicum, in the period from December 2019 to August 2020 on a group of 201 women. A diagnostic survey and the HR-HPV molecular test were used in the research. All of the obtained samples were subjected to identification of and testing for the presence of HR-HPV by the Cobas 4800 platform (Roche Diagnostic©, Basel, Switzerland). We presented the statistically significant relationships between the age of the respondents and the awareness of the impact. The women aged over 43 years old presented the widest spectrum of information concerning HPV-related lesions. Conclusion: Our study highlights the necessity of educating women about the prevention of sexually transmitted infections.