Investigator

Michel Salzet

Distuguish Professor (PRCE2) · Ministère de l'Education nationale de l'Enseignement supérieur et de la Recherche

About

MSMichel Salzet
Papers(3)
Deciphering the ghost…In-depth proteomics a…Fallopian tube lesion…
Collaborators(8)
Tristan CardonSoulaimane AboulouardIsabelle FournierMaxence WisztorskiFiras KobeissyMarie DuhamelXavier RoucouDiego Fernando Garcia…
Institutions(3)
InsermAmerican University O…Université de Sherbro…

Papers

Deciphering the ghost proteome in ovarian cancer cells by deep proteogenomic characterization

Abstract Proteogenomics is becoming a powerful tool in personalized medicine by linking genomics, transcriptomics and mass spectrometry (MS)-based proteomics. Due to increasing evidence of alternative open reading frame-encoded proteins (AltProts), proteogenomics has a high potential to unravel the characteristics, variants, expression levels of the alternative proteome, in addition to already annotated proteins (RefProts). To obtain a broader view of the proteome of ovarian cancer cells compared to ovarian epithelial cells, cell-specific total RNA-sequencing profiles and customized protein databases were generated. In total, 128 RefProts and 30 AltProts were identified exclusively in SKOV-3 and PEO-4 cells. Among them, an AltProt variant of IP_715944, translated from DHX8 , was found mutated (p.Leu44Pro). We show high variation in protein expression levels of RefProts and AltProts in different subcellular compartments. The presence of 117 RefProt and two AltProt variants was described, along with their possible implications in the different physiological/pathological characteristics. To identify the possible involvement of AltProts in cellular processes, cross-linking-MS (XL-MS) was performed in each cell line to identify AltProt-RefProt interactions. This approach revealed an interaction between POLD3 and the AltProt IP_183088, which after molecular docking, was placed between POLD3-POLD2 binding sites, highlighting its possibility of the involvement in DNA replication and repair.

486Works
3Papers
8Collaborators
2Trials
Cell Line, TumorTumor MicroenvironmentOvarian NeoplasmsAntigens, NeoplasmLymphocytes, Tumor-InfiltratingVitisPlant Diseases

Positions

2014–

Distuguish Professor (PRCE2)

Ministère de l'Education nationale de l'Enseignement supérieur et de la Recherche

2010–

Distinguish Professor (PRCE1)

Ministere de l'Education nationale de l'Enseignement superieur et de la Recherche Secretariat de l'Enseignement superieur et de la Recherche

1995–

Full Professor

Ministere de l'Education nationale de l'Enseignement superieur et de la Recherche Secretariat de l'Enseignement superieur et de la Recherche

Education

1995

Habilitation

Université Lille 1

1993

PhD

Université Lille 1

Country

FR

Keywords
proteomicneuroimmulogyMALDI mass SpectrometryNeuroendocrinology