Genetic Polymorphism of miR-218-2 (rs11134527) in Cervical Cancer: A
Case-Control Study on the Bangladeshi Women
Background:
The prevalence of Cervical Cancer (CC) is disproportionately higher in developing
countries. It is the second most frequent cancer type among Bangladeshi women and the
major cause of morbidity and mortality. However, no previous data reported the association of
miR-218-2 gene polymorphisms in Bangladeshi cervical cancer patients.
Aim:
This case-control study was designed to find the link between the rs11134527 polymorphism
in miR-218-2 and CC.
Methods:
A total of 488 subjects were recruited, comprising 256 cervical cancer patients and 232
healthy females. Genotyping was conducted with the tetra-primer ARMS-PCR technique to detect
the association.
Results:
The results of genotype data showed that rs11134527 was in the Hardy-Weinberg equilibrium
in both CC cases and controls (P >0.05). Overall, the polymorphism was found to be significantly
associated with an increased risk of cervical cancer with AG genotype (AG vs. GG: OR =
2.26, 95% Cl = 1.40-3.66, P = 0.0008), AA genotype (AA vs. GG: OR = 3.64, 95% Cl = 2.17-6.10,
P <0.0001), dominant model (AG+AA vs. GG: OR = 2.75, 95% Cl = 1.75-4.31, P <0.0001), recessive
model (AA vs. GG+AG: OR = 2.08, 95% Cl = 1.41-3.08, P = 0.0002), and A allele (A vs. G:
OR = 1.94, 95% Cl = 1.51-2.51, P <0.0001). All of these correlations remained statistically significant
after performing Bonferroni correction (P <0.008).
Conclusion:
Our study suggests that the rs11134527 polymorphism in the miR-218-2 gene contributes
to the susceptibility of CC in Bangladeshi women.
