Liping Zhang

Enhancement of circular RNA 0002577 in serum exosomes in patients with endometrial cancer accelerates disease progression via general transcription factor II-I repeat domain-containing 1 (GTF2IRD1)

Uterine corpus endometrial carcinoma (UCEC) is a common gynecologic malignancy with poor prognosis in advanced stages. Circular RNA (circRNA) and exosomes have been documented as significant contributors to the advancement of tumor cells, but the specific regulatory mechanisms between them is unclear. Therefore, our study attempts to explore the mechanism between them. Firstly, we isolated and identified exosomes, and then validated their role in UCEC progression by experiments in vivo and in vitro. Secondly, a human competing endogenous RNA (ceRNA) array was used to identify the circRNA with the most significant differences in expression from serum of UCEC patient, and validated its role in UCEC progression by experiments in vitro. Then, we find the target gene of this circRNA by RNA sequencing, and further clarify the correlation between those and their role in tumor cell progression through experiments in vitro. Serum exosomes in patients with UCEC can promote the progression of UCEC. The human ceRNA array identified that circRNA 0002577 (circ_0002577) was up-regulated and was the most significantly altered circRNA. Moreover, the up-regulated circ_0002577 in exosomes derived from UCEC patients promote proliferation and migration of UCEC. Based on RNA sequencing results, general transcription factor II-I repeat domain-containing 1 ( Exosomes promote UCEC progression through circ_0002577 mediated regulation of

SRSF3 Restriction Eases Cervical Cancer Cell Viability and Metastasis via Adjusting PI3K/AKT/mTOR Signaling Pathway

Objective. To investigate the effect of SRSF3 on the viability and metastasis of cervical cancer (CC) SiHa and Hela cells. Methods. In vitro, HeLa cells and SiHa cells were cultured. In cervical cancer cells, RNA interference technology was utilized to lessen the SRSF3 level, and via RT‐PCR utilization, the SRSF3 level in every group of cells was revealed. By employing the CCK‐8 method, the OD value was revealed in every group at 24, 48, 72, and 96 h. On the migration of cervical cancer SiHa and HeLa cells via transwell utilizing, the consequence of SRSF3 was surveyed. Through western blotting utilizing, the PI3K/AKT/mTOR signaling pathway‐connected proteins levels was revealed. Results. In SiHa cells, contrasted to the NC‐SiHa group, the SRSF3 level, the number of invasive cells per unit area, the p‐PI3K/PI3K level, the p‐AKT/AKT level, and the p‐mTOR/mTOR level in the si‐SRSF3 group were substantially lessened. The OD value at 490 nm of the si‐SRSF3 group had no impressive divergence, contrasted to the NC‐SiHa group at 24 h. At 48 h, the OD value of the si‐SRSF3 group was impressively lessened than that of the NC‐SiHa group. This connection was time‐dependent. In HeLa cells, the SRSF3 level, the number of invasive cells per unit area, the level of p‐PI3K/PI3K, the level of p‐AKT/AKT, and the level of p‐mTOR/mTOR in the cells of the si‐SRSF3 group in the NC‐HeLa group were impressively lessened than those in the NC‐Hela group. Between the NC‐HeLa group and the si‐SRSF3 group at 24 h, there was no impressive divergence in the OD value at 490 nm. At 48 h, the OD value of the si‐SRSF3 group was impressively lessened than that of the NC‐SiHa group. This connection is time‐dependent. Conclusion. Reducing the SRSF3 level can restrain the viability and metastasis of cervical cancer cells via restraining the PI3K/AKT/mTOR signaling pathway.