Investigator

Lin Lin

Assistant Professor · Aarhus University, Department of Biomedicine

About

LLLin Lin
Papers(2)
Ovarian tumor cells g…Single-cell transcrip…
Collaborators(10)
Yonglun LuoNarsimha MamidiPatrick C. MartinRajan GognaRasmus O. BakRonit Satchi-FainaroSagar RayamajhiSeungpyo HongSteven R. GrossmanUsha Menon
Institutions(7)
Aarhus UniversityUniversity of Wiscons…Cedars-Sinai Medical …Virginia Commonwealth…Tel Aviv UniversityUniversity of Kansas …University College Lo…

Papers

Single-cell transcriptome analysis of epithelial, immune, and stromal signatures and interactions in human ovarian cancer

Abstract A comprehensive investigation of ovarian cancer (OC) progression at the single-cell level is crucial for enhancing our understanding of the disease, as well as for the development of better diagnoses and treatments. Here, over half a million single-cell transcriptome data were collected from 84 OC patients across all clinical stages. Through integrative analysis, we identified heterogeneous epithelial-immune-stromal cellular compartments and their interactions in the OC microenvironment. The epithelial cells displayed clinical subtype features with functional variance. A significant increase in distinct T cell subtypes was identified including Tregs and CD8+ exhausted T cells from stage IC2. Additionally, we discovered antigen-presenting cancer-associated fibroblasts (CAFs), with myofibroblastic CAFs (myCAFs) exhibiting enriched extracellular matrix (ECM) functionality linked to tumor progression at stage IC2. Furthermore, the NECTIN2-TIGIT ligand-receptor pair was identified to mediate T cells communicating with epithelial, fibroblast, endothelial, and other cell types. Knock-out of NECTIN2 using CRISPR/Cas9 inhibited ovarian cancer cell (SKOV3) proliferation, and increased T cell proliferation when co-cultured. These findings shed light on the cellular compartments and functional aspects of OC, providing insights into the molecular mechanisms underlying stage IC2 and potential therapeutic strategies for OC.

47Works
2Papers
35Collaborators
Tumor MicroenvironmentCell Line, TumorOvarian NeoplasmsAnaerobiosisColorectal NeoplasmsNeoplasm InvasivenessPhagocytosis

Positions

2016–

Assistant Professor

Aarhus University · Department of Biomedicine

2012–

Post doc

Aarhus University · Department of Biomedicine

2011–

Vice general and scientific manager

BGI · BGI Ark Biotechnology Co., Ltd

2010–

Scientific directo

BGI · BGI Ark Biotechnology Co., Ltd

Education

2010

PhD

Aarhus University

2007

Master

Chinese Academy of Sciences · Beijing Insititute of Genomics

2006

Bachelor

China Agricultural University · Honors Program in Life Science