Homa Soleimani

In vitro Evaluation of the Combined Effects of a Static Magnetic Field and Curcumin on MCF7 and HeLa Cell Lines

This study examined how varying levels of curcumin and static magnetic fields (SMF) affected three cell types, with outcomes influenced by field strength and curcumin concentration in normal and cancerous cells. The effects of static magnetic fields on cell proliferation and death rates were evaluated using the MTT assay and flow cytometry. The efficacy of the magnetic field, with or without curcumin (10-40 µg/ml), was assessed. Cells were treated with curcumin at the optimal concentration from the MTT assay and simultaneously exposed to a static magnetic field (7, 10, or 25 mT) for 48 hours. Our study demonstrated that SMF (7 mT) exposure significantly increased the proportion of HeLa and MCF-7 cells in the early apoptotic phase. Curcumin application markedly elevated necrosis rates in both cell lines. Additionally, curcumin (5 µg/ml) significantly affected apoptosis rates in HeLa and MCF-7 cells (p < 0.05). This study demonstrated that SMF exposure significantly increased necrotic cell death in HeLa cells and accelerated apoptosis in both cancer cell types. The minimum effective dose of curcumin combined with SMF caused a four-fold increase in apoptosis in HeLa cells compared to curcumin alone.

Influence of Static Magnetic Field on HeLa and Huo2 Cells in the Presence of Aloe vera Extract

This research aimed to assess the impact of static magnetic field (SMF) on apoptosis rate and cell cycle progression in the presence of Aloe vera Crude Extract (ACE) in normal (Huo2) and cancer cells (HeLa). The specimens were split into one untreated group (control) and two experimental groups, including treatment with ACE (Alo) and compound treatment with SMF and ACE (Alo+SMF). MTT assay determined the IC50 value, and flow cytometry was employed to evaluate cell cycle distribution and apoptosis rates. Statistical analysis was carried out through a two-way ANOVA followed by Tukey's post hoc test. Our results showed that combination treatment with SMF (10 mT) and ACE (Alo+SMF) significantly inhibited the cell proliferation. This increased the cell number in G2/M stage and early apoptosis in cancer cells compared to ACE treated cells after 24 and 48h but reduced the number of Huo2 cells in G2/M phase and early apoptosis after 24h. The effect of AEC on HeLa cells was intensified with increasing the SMF exposure time, such that the early apoptosis rate in Alo+SMF group had an approximate 4-fold increase compared to Alo group. This research proposes that the combination treatment accelerates the apoptosis induction of HeLa cell. During the interphase, there were significant differences between the cancer and healthy cells concerning the cell cycle. Moreover, exposure time may play an important role in the impact SMF on both healthy and cancer cells in the presence of AEC.