Investigator

Deusdedit Tusubira

Lecturer · Mbarara University of Science and Technology Faculty of Medicine, Biochemistry

DTDeusdedit Tusubira
Papers(1)
Diagnostic Accuracy o…
Collaborators(10)
Doreen NuwashabaFrank SsedyabaneHakho LeeJosephine Nambi Najju…Joseph NgonziNixon NiyonzimaRogers KajabwanguThomas C. RandallAlexcer NamuliCesar Castro
Institutions(4)
Mbarara University Of…Harvard Medical SchoolUganda Cancer Institu…Columbia University M…

Papers

Diagnostic Accuracy of Serum P16ink4A and FOX‐P3 Concentrations for Detection of Cervical Lesions Among Women Attending a Cervical Cancer Clinic in Western Uganda: A Case‐Control Study

Introduction: Expression of P16ink4A and FOXP3 is correlated with the grades of cervical lesions. In this study, we determined the diagnostic accuracy of serum P16ink4A and FOXP3 concentrations for detection of cervical intraepithelial neoplasia (CIN) and cervical cancer (CC) in a rural setting in Southwestern Uganda. Material and Methods: CIN and CC cases (93 each before treatment), and 93 controls were identified. Clinical and demographic data were documented before quantifying serum P16ink4A and FOXP3 concentrations using quantitative ELISA kits. Cases were confirmed by cytology and/or histology. We employed descriptive statistics, cross‐tabulation, and receiver operating curves (ROC) using statistical software for data science (STATA) 17.  p ‐values <0.05 were considered statistically significant. Results: Serum FOXP3 concentration of 0.0545 ng/mL < showed moderate sensitivity (32.22% and 57.78%) for detection of CIN and CC from healthy controls, respectively. It also showed a moderately high specificity of 68.89% for detection of both CIN and CC from healthy controls (AUC‐0.6014 and 0.7679, respectively). Serum P16ink4A concentration of 0.946 ng/mL < showed moderate sensitivities (50.00% and 60.00%) and specificities (56.67% and 55.56%) for the detection of CIN and CC from healthy controls, respectively (AUC‐0.6085 and 0.7592, respectively). A combination of elevated serum FOXP3 and P16ink4A showed very low sensitivities of 18.89% in detecting CIN from healthy controls and 33.33% for detecting CC from healthy controls. This combination showed high specificity of 83.33% in detecting both CIN and CC from healthy controls (AUC‐0.5992 and 0.7642, respectively). Conclusion: Although serum P16ink4A and FOXP3 concentrations showed moderate accuracy, their combination was more specific than sensitive. This combination has a high potential to be applied for diagnosis rather than screening for cervical lesions, at least in the Ugandan population. Combinations of P16ink4A and FOXP3 with other biomarkers could improve diagnostic accuracies. Additionally, studies could be conducted to assess the performance of these biomarkers in the detection of cervical lesions in specific populations, say Human Immunodeficiency Virus (HIV)‐positive and HIV‐negative populations.

41Works
1Papers
11Collaborators
Biomarkers, TumorNeoplasmsUterine Cervical NeoplasmsPrognosis

Positions

2002–

Lecturer

Mbarara University of Science and Technology Faculty of Medicine · Biochemistry

2001–

Medical Sales Representative

Star Pharmaceuticals · Business Development

2000–

Research Assiatant

Makerere University · Chemistry

Education

2019

PhD

University of Bergen · Biomedicine

2005

Master of Science Molecular Biology of parasites and disease vector

Liverpool School of Tropical Medicine

2001

Bachelor of Science

Makerere University · Biochemistry and sports science

Country

UG

Keywords
Age-related disordersnon-communicable diseasesnon-communicable diseasesAlzheimer's disease and Alzheimer related disordersAlzheimer's disease and Alzheimer related disordersdiabetesNatural productsMatebolism and metabolic related disordersprostate hyperplasia
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