Investigator

Changsun Choi

Professor · Chung-Ang University, Department of Food and Nutrition

CCChangsun Choi
Papers(1)
Piperlongumine induce…
Collaborators(1)
Ji-Youn Jung
Institutions(2)
Chung Ang UniversityUnknown Institution

Papers

Piperlongumine induces apoptosis and autophagy via the PI3K/Akt/mTOR pathway in KB human cervical cancer cells

Natural products are continuously being researched to develop safe and effective treatment options for cervical cancer, the fourth most common cancer in women. Piperlongumine (PL), an amide alkaloid mainly present in long pepper, exhibits neuroprotective and anti-cancer properties. However, the specific effect of PL in cervical cancer and the relationship between the anti-cancer pathway and autophagy remain unclear. Therefore, we aimed to investigate PL-induced apoptosis in KB human cervical cancer cells and the relationship between apoptosis and autophagy therein. 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and wound-healing assays showed that PL treatment suppressed KB cell viability and proliferation. Apoptosis was identified through 4',6-diamidino-2-phenylindole and annexin V-propidium iodide staining, increased cleaved-poly (ADP-ribose) polymerase and Bcl-2 associated X levels, and decreased B cell lymphoma 2 levels. Acridine orange staining and increased microtubule-associated protein 1A/1B-light chain 3-II and Beclin-1 levels confirmed autophagy. We determined that KB cell-related autophagy exerted cytoprotective effects using the autophagy inhibitors 3-methyladenine and hydroxychloroquine. PL treatment promoted apoptosis by inhibiting the phosphatidylinositol-3-kinase (PI3K)/protein kinase B/mammalian target of rapamycin pathway in KB cells; inhibiting the pathway using PI3K inhibitors increased autophagy. We suggest that PL is a potential natural anticancer agent for cervical cancer treatment.

207Works
1Papers
1Collaborators

Positions

2005–

Professor

Chung-Ang University · Department of Food and Nutrition

Education

2003

PhD

Seoul National University · Veterinary Medicine

2000

Master degree

Seoul National University · Veterinary Medicine

1996

Bachelor degree / D.V.M.

Seoul National University · Veterinary Medicine

Country

KR

Keywords
NorovirusFoodborne virusDetectionControlAntiviral
Links & IDs
0000-0001-7730-8538

Scopus: 56804203600

Researcher Id: D-9298-2013