Bo Hu

The Immunotherapeutic Effect of SIRP α ‐Silenced DCs against Cervical Cancer

Signal regulatory protein α (SIRP α ), a transmembrane protein that is predominantly expressed in dendritic cells (DCs) or macrophages, interacts with CD47 that is overexpressed in almost all types of tumor cells. The interaction between SIRP α and CD47 leads to a negative signal that prevents the phenotypic and functional maturation of DC and inhibits phagocytosis. The SIRP α knockdown in DCs that were pulsed with a modified HPV16E7 (HPV16mE7) protein with enhanced antigenicity and reduced transformation activity results in increased cytokine (TNF‐ α /IL‐12/IL‐6) secretion, IFN‐ γ secretion by T lymphocytes, and in vitro / in vivo tumoricidal activity against cervical cancer cells. Taken together, these results suggest that SIRP α ‐silenced DC vaccination presented potential therapeutic implications against cervical cancer.