Pelvic Microbiomes of Benign and Malignant Ovarian Diseases

NCT03388996UNKNOWNOBSERVATIONAL

Summary

This case-control study aims to compare the pelvic microbiomes of benign ovarian diseases and ovarian malignancies by 16s RNA techniques and culture. Discharges/flushing fluid from vagina, faces and fimbria end of fallopian tube are collected from age and menopausal status matched patient before and during procedures of laparoscopies. The discharges/flushing fluid would be sent for 16s RNA analysis and microculture respectively, and the results would get self-contrasted comparison and case-control comparison.

Key Facts

Lead Sponsor

Lei Li

Enrollment

40

Start Date

2018-01-01

Completion Date

2020-01-01

Study Type

OBSERVATIONAL

Official Title

The Role of Pelvic Microbiomes During the Malignant Transformation of Benign Ovarian Diseases

Interventions

microbiomes

Conditions

MicrobiotaOvarian NeoplasmsRibosome Alteration

Eligibility

Sex

FEMALE

Inclusion Criteria:

* Laparoscopy procedures
* Specific pathological diagnosis

Exclusion Criteria:

* Previous history of pelvic surgeries
* Evidences of pelvic inflammation diseases

Outcome Measures

Primary Outcomes

Difference of species distribution of microorganism according to 16s RNA analysis

The difference of species of microorganism distribution according to 16s RNA sequencing analysis between control group and study group, and between different sampling sites in each arm

Time frame: 2 years

Difference of species distribution of microorganism according to microculture outcomes

Difference of species distribution of microorganism according to microculture outcomes between control group and study group, and between different sampling sites in each arm

Time frame: 2 years

Locations

Peking Union Medical College Hospital, Beijing, China

Linked Diseases

Ovarian Neoplasms

Ovarian Neoplasms — a type of gynecological cancer.

Pelvic Microbiomes of Benign and Malignant Ovarian Diseases